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AL-Diagnostic GmbH

We carry on the outstanding precast gels from Elchrom Scientific - we set quality standards in DNA/RNA electrophoresis and oligonucleotide purification. The ready-to-use gels are based on a novel matrix and are ideal for a wide range of applications in plant, human and animal genomics.

The distribution of our precast gels is exclusively carried out by AL-Labortechnik.


Product Overview:

DNA/RNA-Precast Gels:

Optimal Size Range in bp  Resolution

Spreadex®

50-1000    1 - 4 bp     
    EL 1200 250-800    4 bp
    EL 800 200-500    4 bp
    EL 600 150-350    4 bp
    EL 400 65-250    1 bp*
PCR CheckIT™ 100-4000     10 bp
GMA 100-450     1 bp
Poly(NAT)® 50-20000    

   2 -3%**

    9 % 180-800 
   12 % 60-300 
Short 
Fragment™
   <50     1 bp/nt

Equipement:

Electrophoresis System
Electr. Equipment
Consumables
Reagents

 

Poly(NAT)® Broadest Size Range

Poly(NAT)® gels are the best choice for demanding separations, covering the broadest range of fragment sizes.They cover DNA fragments in the size range from about 50 bp to 20000 bp and are able to resolve 2–3 % in size difference for fragments up to 2 kb.


Poly(NAT)® is prepared by the polymerization of NAT (N-acryloyl-tris(hydroxymethyl)-aminomethane) in the presence of a cross-linker.Electrophoresis of DNA on Poly(NAT)® gels can be carried out over a wide temperature range from 20°C to 55°C.
Poly(NAT)® gels do not melt at high temperature (95°C). Electrophoresis at 55°C is recommended for precise DNA size-estimations because sequence-dependent mobility of DNA is mostly prevented at elevated temperatures.

Poly(NAT)® gels are highly transparent. SYBR-Gold staining allows to detect less than 0.1 ng DNA per band.
Poly(NAT)® gels provide exceptionally clear and sharp bands.
Poly(NAT)® gels exist in 3 different concentrations (6%, 9%, 12%). Which type of Poly(NAT)® gel to choose depends on the size range of the DNA fragments to be analysed (see ELQuant for detailed information).
Diffusion of DNA in Poly(NAT)® gels is extremely slow, even after electrophoresis.
Immediate staining and destaining is not required.

Poly(NAT)® Special Features

  • Broad fragment size range: from 50 bp - 20`000 bp
  • High resolution: 2–3 %, up to 2 kb
  • High sensitivity: as little as 0.05 ng/band
  • Electrophoresis temperature range: 20 – 55°C
  • Low diffusion: bands remain sharp after weeks at 4°C
  • Direct recovery: stained bands can be excised and used directly for PCR, sequencing and cloning


Poly(NAT)® General Notes

  • Poly(NAT)® gels exist in 3 different concentrations: 6%, 9%, 12%
  • Always use 30 mM TAE buffer with Poly(NAT)® gels. 
  • Borate is not compatible with Poly(NAT)®
  • Stain after electrophoresis
  • Storage: horizontally at 4°C (40°F), do not freeze!
  • Shelf life: at least 3 months (max. 6 months)

Ordering Information

       
Description Order number Qantity Price Shop
12% Poly(NAT)® Mini 3012 1 Box of 10 Gels  
9% Poly(NAT)® Wide Mini S-2x25 3049 1 Box of 6x2 Gels  
 Für Probegele kontaktieren sie bitte unser Office: 07472/ 234 230 oder This email address is being protected from spambots. You need JavaScript enabled to view it. - For samples please contact This email address is being protected from spambots. You need JavaScript enabled to view it.
    

 

Different Formats:

AL's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel. AL-Diagnostic provides two main formats of gels:

1) Mini Gels

2) Wide Mini Gels -  2 or 4 gels per presentation.

 Mini 8/12 wells
 

Running distance:
87 mm  for 8 samples
57 mm for 12 samples

Wide Mini 2x13 and 4x13

Running distance:
87 mm for 2x13
40 mm for 4x13

Wide Mini 2x25 and 4x25

Running distance:
87 mm for 2x25
40 mm for 4x25

ahn

brand

eppendorf

gilson

mettler toledo

heidolph

neolab

satorius

systec

thermo scientific

zeiss