Spreadex® - Für beste Auflösung im Bereich 50 bp bis 1000 bp
High Resolution Spreadex® for sensational resolution:
Spreadex® gels provide highest resolution power in the size range of 50 bp to 1000 bp. The resolving power is three times higher than the resolving power of any other synthetic gel matrix.
The synthetic polymers used for Spreadex® form an unique three-dimensional structure in which the polymers meet the migrating DNA fragments with increasing resistance. Selective retardation of long DNA molecules results in larger spacing between fragments. The bands become more spread out along the gel length, but remain sharp, even at high running temperatures.
Spreadex® gels do not melt at high temperatures (95°C) and do not inhibit DNA polymerases and ligases.
Consequently, bands identified by a conventional dye can be excised with BandPick™ and used directly for reamplification, sequencing or cloning.
Each Spreadex® gel is characterized by an exclusion limit (EL). DNA molecules longer than the indicated EL do not migrate in the gel even after prolonged electrophoresis. The gels are available in six different exclusion limits (EL 300, 400, 500, 600, 800, 1200).
Spreadex® EL 300 and EL 400 gels resolve fragments of only 1 bp difference for a total fragment length of up to about 150 bp. DNA fragments differing by 4 bp are easily resolved on all Spreadex® gels.
The synthetic polymers used for Spreadex® form an unique three-dimensional structure in which the polymers meet the migrating DNA fragments with increasing resistance. Selective retardation of long DNA molecules results in larger spacing between fragments. The bands become more spread out along the gel length, but remain sharp, even at high running temperatures.
Spreadex® gels do not melt at high temperatures (95°C) and do not inhibit DNA polymerases and ligases.
Consequently, bands identified by a conventional dye can be excised with BandPick™ and used directly for reamplification, sequencing or cloning.
Each Spreadex® gel is characterized by an exclusion limit (EL). DNA molecules longer than the indicated EL do not migrate in the gel even after prolonged electrophoresis. The gels are available in six different exclusion limits (EL 300, 400, 500, 600, 800, 1200).
Spreadex® EL 300 and EL 400 gels resolve fragments of only 1 bp difference for a total fragment length of up to about 150 bp. DNA fragments differing by 4 bp are easily resolved on all Spreadex® gels.
PCR CheckIT™ Gele - Fast runs and easy DNA recovery
PCR CheckIT™ gels are ideal for fast screening in the size range from 100 bp to 4 kbp.
The gels are produced by gelation and simultaneous cross-linking of the hydroxyl groups of agarose and the oxirane groups of butanediol diglycidylether. They contain a low total polymer concentration, but give improved resolution of short nucleic acid fragments. DNA molecules run faster in PCR CheckIT™ gels than in high percent agarose or fully synthetic gels.
PCR CheckIT™ gels are insoluble in boiling water and do not inhibit DNA polymerases and ligases. The gel is elastic and optically clear. The stained bands from a gel can be excised with BandPick™ and used directly for re-amplification,
sequencing or cloning.
PCR CheckIT™ gels are available with or without Ethidium Bromide (EtBr). The samples can be run in the presence of EtBr to achieve excellent results. PCR CheckIT™ gels are not required to be destained prior to analysis and results can be obtained within 10 minutes.
The gels are produced by gelation and simultaneous cross-linking of the hydroxyl groups of agarose and the oxirane groups of butanediol diglycidylether. They contain a low total polymer concentration, but give improved resolution of short nucleic acid fragments. DNA molecules run faster in PCR CheckIT™ gels than in high percent agarose or fully synthetic gels.
PCR CheckIT™ gels are insoluble in boiling water and do not inhibit DNA polymerases and ligases. The gel is elastic and optically clear. The stained bands from a gel can be excised with BandPick™ and used directly for re-amplification,
sequencing or cloning.
PCR CheckIT™ gels are available with or without Ethidium Bromide (EtBr). The samples can be run in the presence of EtBr to achieve excellent results. PCR CheckIT™ gels are not required to be destained prior to analysis and results can be obtained within 10 minutes.
GMA - SSCP und Heteroduplexanalyse
Optimized to screen Point Mutations
Possible methods include: Single Strand Conformation Polymorphism (SSCP) and Heteroduplex Analysis.
The SSCP method relies on the different migration rates of single stranded DNA fragments in a non-denaturing gel run at low temperature. Two single stranded DNA fragments that differ in just one nucleotide often migrate at rates that are sufficiently different to allow complete separation of the two fragments.
For successful SSCP, the length of the DNA fragment should be in the range of 150 bp to 350 bp and the electrophoresis should be performed at constant temperature in the range of 5°C to 20°C. The Elchrom electrophoresis system combined with GMA gels is the best choice to achieve excellent results in SSCP and heteroduplex analysis.
The SSCP method relies on the different migration rates of single stranded DNA fragments in a non-denaturing gel run at low temperature. Two single stranded DNA fragments that differ in just one nucleotide often migrate at rates that are sufficiently different to allow complete separation of the two fragments.
For successful SSCP, the length of the DNA fragment should be in the range of 150 bp to 350 bp and the electrophoresis should be performed at constant temperature in the range of 5°C to 20°C. The Elchrom electrophoresis system combined with GMA gels is the best choice to achieve excellent results in SSCP and heteroduplex analysis.
Poly(NAT)® - Fine separations in broad size ranges
Poly(NAT)® Broadest Size Range
Poly(NAT)® gels are the best choice for demanding separations, covering the broadest range of fragment sizes.They cover DNA fragments in the size range from about 50 bp to 20000 bp and are able to resolve 2–3 % in size difference for fragments up to 2 kb.
Poly(NAT)® is prepared by the polymerization of NAT (N-acryloyl-tris(hydroxymethyl)-aminomethane) in the presence of a cross-linker.Electrophoresis of DNA on Poly(NAT)® gels can be carried out over a wide temperature range from 20°C to 55°C.
Poly(NAT)® gels do not melt at high temperature (95°C). Electrophoresis at 55°C is recommended for precise DNA size-estimations because sequence-dependent mobility of DNA is mostly prevented at elevated temperatures.
Poly(NAT)® gels are highly transparent. SYBR-Gold staining allows to detect less than 0.1 ng DNA per band.
Poly(NAT)® gels provide exceptionally clear and sharp bands.
Poly(NAT)® gels exist in 3 different concentrations (6%, 9%, 12%). Which type of Poly(NAT)® gel to choose depends on the size range of the DNA fragments to be analysed (see ELQuant for detailed information).
Diffusion of DNA in Poly(NAT)® gels is extremely slow, even after electrophoresis.
Immediate staining and destaining is not required.
Poly(NAT)® is prepared by the polymerization of NAT (N-acryloyl-tris(hydroxymethyl)-aminomethane) in the presence of a cross-linker.Electrophoresis of DNA on Poly(NAT)® gels can be carried out over a wide temperature range from 20°C to 55°C.
Poly(NAT)® gels do not melt at high temperature (95°C). Electrophoresis at 55°C is recommended for precise DNA size-estimations because sequence-dependent mobility of DNA is mostly prevented at elevated temperatures.
Poly(NAT)® gels are highly transparent. SYBR-Gold staining allows to detect less than 0.1 ng DNA per band.
Poly(NAT)® gels provide exceptionally clear and sharp bands.
Poly(NAT)® gels exist in 3 different concentrations (6%, 9%, 12%). Which type of Poly(NAT)® gel to choose depends on the size range of the DNA fragments to be analysed (see ELQuant for detailed information).
Diffusion of DNA in Poly(NAT)® gels is extremely slow, even after electrophoresis.
Immediate staining and destaining is not required.
Different Formats
AL's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel. AL-Diagnostic provides two main formats of gels:
1) Mini Gels
2) Wide Mini Gels - 2 or 4 gels per presentation.