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PCR Enzyme

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Product Overview:

Ampilqon
Analytik Jena
Molzym
>
Standard PCR Reagents
>
DNA-free PCR Reagents

 

MolTaq 16S/18S and Mastermix 16S/18S

There is a strong risk of false positive signals as a cause of amplification from contaminating DNA in PCR reagents, including the polymerising enzyme, primers and dNTPs.

Molzym’s purification technology removes traces of DNA from the PCR reagents and supplies a highly active enzyme in the products. This results in enhanced sensitivity and reliability of bacteria and fungi detection in the assays.

Solutions are offered to meet your requirements ranging from DNA-free Taq polymerases, MolTaq 16S/18S and Hot MolTaq 16S/18S to master mixes in its various designs, Mastermix 16S and Mastermix 18S kits. Assays are available, Mastermix 16S Primer and Mastermix 16S Complete, for the detection of bacterial DNA, and Mastermix 18S Primer and Mastermix 18S Complete, for the detection of fungal DNA by the amplification of a highly variable regions of the 16S and 18S rRNA gene, respectively.

If you want to use your own primers, we offer Mastermix 16S/18S Basic and Mastermix 16S/18S Dye kits which contain all components for the PCR reaction except primers.

Sequencing of amplicons combined with online homology BLAST search is an option for the identification of bacteria and fungi detected by Molzym assays, Mastermix16S/18S Complete or Mastermix 16S/18S Primer. Molzym offers sequencing primers that allow the sequence analysis and identification of bacteria and fungi. The primers bind to conserved regions within the amplified DNA enabling sequencing of highly discriminative sequences for strain identification. Sequencing primer SeqGP16 covers a variety of Gram-positive organisms, including staphylococci, streptococci and enterococci. Sequencing primer SeqGN16 on the other hand allows the identification of many pathogenic Gram-negative bacteria, including enterobacteria and pseudomonads.

Product Overview:

Product Application
MolTaq 16S/18S Taq for bacterial and fungal DNA detection
Hot MolTaq 16S/18S Hot start Taq for bacterial and fungal DNA detection
Mastermix 16S/18S Basic custom PCR assays
Mastermix 16S/18S Dye custom Real-Time PCR assays
Mastermix 16S Primer eubacterial PCR assay
Mastermix 18S Primer panfungal PCR assay
Mastermix 16S Complete eubacterial Real-Time PCR assay
Mastermix 18S Complete panfungal Real-Time PCR assay
Application Custom primers Eubacterial assays
Panfungal assays
Product Mastermix 16S/18S Mastermix 16S Mastermix 18S
Basic Dye Primer
Complete
Primer
Complete
MolTaq 16S/18S, buffer, water + + + + + +
dNTPs + + + + + +
Fluorescing dye - + - + - +
Universal 16S primers - - + + - -
Universal 18S primers - - - - + +

 

 

DNA-free water is high quality PCR-grade water that is used in the setup of PCR and Real-Time PCR assays demanding high accuracy of analysis. Applications comprise quality control of manufacturing processes in pharma and biotechnology, contamination control of food products, environmental analysis, molecular infection diagnosis, SNP and other hereditary mutation analyses, and research. DNA-free water allows the precise molecular analysis with utmost reliability. This product is manufactured under strict quality control management guaranteeing the absence of human and microbial DNA contamination (<3% false-positive rate).

Product Content
DNA-free water, PCR-grade DNA-free water, 10x 1.7ml 

Sequencing Primers in conjunction with eubacterial PCR assays (Mastermix 16S Primer and Mastermix 16S Complete) and panfungal PCR assays (Mastermix 18S Primer and Mastermix 18S Complete):

Product Content Application
Set of sequencing primers eubacterial SeqGP16 and SeqGN16 (10pmol/µl) sequencing of amplicons
Sequencing primer panfungal SeqYeast (10pmol/µl) sequencing of amplicons

ahn

brand

eppendorf

gilson

mettler toledo

heidolph

neolab

satorius

systec

thermo scientific

zeiss