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AL-Diagnostic GmbH

We set new quality standards in DNA/RNA electrophoresis and oligonucleotide purification. The ready-to-use gels are based on a novel matrix and are ideal for a wide range of applications in plant, human and animal genomics.

The distribution of our precast gels is exclusively carried out by AL-Labortechnik.


Product Overview:

DNA/RNA-Precast Gels:

Optimal Size Range in bp  Resolution

Spreadex®

50-1000    1 - 4 bp     
    EL 1200 250-800    4 bp
    EL 800 200-500    4 bp
    EL 600 150-350    4 bp
    EL 400 65-250    1 bp*
PCR CheckIT™ 100-4000     10 bp
GMA 100-450     1 bp
Poly(NAT)® 50-20000    

   2 -3%**

    9 % 180-800 
   12 % 60-300 
Short 
Fragment™
   <50     1 bp/nt

Equipement:

Electrophoresis System
Electr. Equipment
Consumables
Reagents

 

Optimized to screen Point Mutations

Possible methods include: Single Strand Conformation Polymorphism (SSCP) and Heteroduplex Analysis.
The SSCP method relies on the different migration rates of single stranded DNA fragments in a non-denaturing gel run at low temperature. Two single stranded DNA fragments that differ in just one nucleotide often migrate at rates that are sufficiently different to allow complete separation of the two fragments.
For successful SSCP, the length of the DNA fragment should be in the range of 150 bp to 350 bp and the electrophoresis should be performed at constant temperature in the range of 5°C to 20°C. The Elchrom electrophoresis system combined with GMA gels is the best choice to achieve excellent results in SSCP and heteroduplex analysis.

GMA Special Features

  • High sensitivity: GMA gels can be stained with SYBR
  • Excellent result: GMA gels are specially developed for SSCP (SNPs, Point Mutation Screening) and heteroduplex analysis
  • High throughput: up to 100 samples analysed at once


GMA General Notes

  • Always use TAE buffer with GMA gels. Borate is not compatible with GMA!
  • Stain after electrophoresis
  • Storage: horizontally at 4°C (40°F)
  • Shelf life: at least 3 months (max. 6 months)

GMA Ordering Information:

Order numberDeascriptionQuantityPriceShop
3526 GMA Wide Mini S-2x13 1 box of 6x2 gels  
3548 GMA Wide Mini S-2x25 1 box of 6x2 gels  

Different Formats:

AL's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel. AL-Diagnostic provides two main formats of gels:

1) Mini Gels

2) Wide Mini Gels -  2 or 4 gels per presentation.

Mini 8/12 wells
 

Running distance:
87 mm  for 8 samples
57 mm for 12 samples

Wide Mini 2x13 and 2x25

Running distance:
87 mm for 2x13
87 mm for 2x25

ahn

brand

eppendorf

gilson

mettler toledo

heidolph

neolab

satorius

systec

thermo scientific

zeiss