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AL-Diagnostic GmbH

We set new quality standards in DNA/RNA electrophoresis and oligonucleotide purification. The ready-to-use gels are based on a novel matrix and are ideal for a wide range of applications in plant, human and animal genomics.

The distribution of our precast gels is exclusively carried out by AL-Labortechnik.


Product Overview:

DNA/RNA-Precast Gels:

Optimal Size Range in bp  Resolution

Spreadex®

50-1000    1 - 4 bp     
    EL 1200 250-800    4 bp
    EL 800 200-500    4 bp
    EL 600 150-350    4 bp
    EL 400 65-250    1 bp*
PCR CheckIT™ 100-4000     10 bp
GMA 100-450     1 bp
Poly(NAT)® 50-20000    

   2 -3%**

    9 % 180-800 
   12 % 60-300 
Short 
Fragment™
   <50     1 bp/nt

Equipement:

Electrophoresis System
Electr. Equipment
Consumables
Reagents

 

High Resolution

Spreadex® for sensational resolution:

Spreadex® gels provide highest resolution power in the size range of 50 bp to 1000 bp. The resolving power is three times higher than the resolving power of any other synthetic gel matrix.
The synthetic polymers used for Spreadex® form an unique three-dimensional structure in which the polymers meet the migrating DNA fragments with increasing resistance. Selective retardation of long DNA molecules results in larger spacing between fragments. The bands become more spread out along the gel length, but remain sharp, even at high running temperatures.
Spreadex® gels do not melt at high temperatures (95°C) and do not inhibit DNA polymerases and ligases.
Consequently, bands identified by a conventional dye can be excised with BandPick™ and used directly for reamplification, sequencing or cloning.
Each Spreadex® gel is characterized by an exclusion limit (EL). DNA molecules longer than the indicated EL do not migrate in the gel even after prolonged electrophoresis. The gels are available in six different exclusion limits (EL 300, 400, 500, 600, 800, 1200).
Spreadex® EL 300 and EL 400 gels resolve fragments of only 1 bp difference for a total fragment length of up to about 150 bp. DNA fragments differing by 4 bp are easily resolved on all Spreadex® gels.

Special Features of Spreadex®

  • Sensational resolution: down to1 bp on 8 cm running distance, 4 bp on 4 cm running distance
  • High throughput: analysing up to 2500 samples per day
  • Direct recovery: identified bands can be excised and directly used for PCR, sequencing and cloning
  • Accurate sizing: DNA runs fragment size-dependent only. Sequence-dependent mobilities are largely eliminated during high temperature electrophoresis (55°C)
  • Zooming: zoom in on your fragment size of interest by choosing the desired exclusion limit
  • High sensitivity: since bands are sharp and highly focused, very little DNA is required per band for detection


Spreadex® General Notes

  • Spreadex® exists in six different exclusion limits (EL): EL 300, 400, 500, 600, 800, 1200. All molecules longer than the indicated EL mentioned above do not enter the gel.
  • Always use 30 mM TAE buffer. Borate is not compatible with Spreadex®.
  • Stain after electrophoresis
  • Storage: horizontally at 4°C (40°F), do not freeze!
  • Shelf life: at least 3 months (max. 6 months)

Precast Gels Overview: Size Range & Resolution:

Type of Gel Optimal Size Range 
bp
  Resolution       Special 
       Features

Spreadex®

  50-1000    1 - 4 bp     

       highest
       resolving 
       power

      
    EL 1200   250-800    4 bp
    EL 800   200-500    4 bp
    EL 600   150-350    4 bp
    EL 400   65-250    1 bp*

Order Information

       
Descriptin Order number Quantity Price Shop
Spreadex EL 400 Mini 3414 1 Box of 10 Gels  
Spreadex EL 600 Mini 3416 1 Box of 10 Gels  
Spreadex EL 800 Mini 3417 1 Box of 10 Gels  
Spreadex EL 1200 Mini 3419 1 Box of 10 Gels  
Spreadex EL 400 Wide Mini S-2x13 3427 1 Box of 6x2 Gels  
Spreadex EL 600 Wide Mini S-2x13 3429 1 Box of 6x2 Gels  
Spreadex EL 800 Wide Mini S-2x13 3430 1 Box of 6x2 Gels  
Spreadex EL 1200 Wide Mini S-2x13 3431 1 Box of 6x2 Gels  
Spreadex EL 400 Wide Mini S-2x25  3443  1 Box of 6x2 Gels  
Spreadex EL 600 Wide Mini S-2x25  3445  1 Box of 6x2 Gels  
Spreadex EL 800 Wide Mini S-2x25  3446  1 Box of 6x2 Gels    
Spreadex EL 1200 Wide Mini S-2x25  3447  1 Box of 6x2 Gels    
Spreadex EL 400 Wide Mini S-4x13   3453  1 Box of 6x4 Gels    
Spreadex EL 600 Wide Mini S-4x13  3455  1 Box of 6x4 Gels    
Spreadex EL 800 Wide Mini S-4x13  3456  1 Box of 6x4 Gels    
Spreadex EL 1200 Wide Mini S-4x13  3457  1 Box of 6x4 Gels    
Spreadex EL 400 Wide Mini S-4x25   3493  1 Box of 6x4 Gels    
 Spreadex EL 600 Wide Mini S-4x25  3495  1 Box of 6x4 Gels    
 Spreadex EL 800 Wide Mini S-4x25  3496  1 Box of 6x4 Gels    
 Spreadex EL 1200 Wide Mini S-4x25  3497  1 Box of 6x4 Gels    
Bezüglich Probegele kontaktieren Sie bitte unser Office unter 07472/234 230 oder This email address is being protected from spambots. You need JavaScript enabled to view it. - For samples please contact This email address is being protected from spambots. You need JavaScript enabled to view it.

Different Formats:

AL's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel. AL-Diagnostic provides two main formats of gels:

1) Mini Gels

2) Wide Mini Gels -  2 or 4 gels per presentation.

Mini 8/12 wells
 

Running distance:
87 mm  for 8 samples
57 mm for 12 samples

Wide Mini 2x13 and 4x13

Running distance:
87 mm for 2x13
40 mm for 4x13

Wide Mini 2x25 and 4x25

Running distance:
87 mm for 2x25
40 mm for 4x25

ahn

brand

eppendorf

gilson

mettler toledo

heidolph

neolab

satorius

systec

thermo scientific

zeiss