Kits - Pathogen DNA Isolation - Molzym
Removal of human DNA | Removal of PCR inhibitors | Sensitivity increase up to factor 40,000 | Broad-range lysis of Gram-positive, Gram-negative bacteria and fungi | DNA-free reagents | Low hands-on-time
MolYsis™ is a pre-analytical tool serving the molecular detection of bacteraemia, fungemia and other infections. MolYsis constitutes of a unique system for the targeted isolation of microbial DNA from clinical samples. By the selective lysis of human cells and the degradation of non-target human DNA, PCR analysis of pathogens is extremely enhanced through increased sensitivity and specificity.
The result of MolYsis pre-treatment with concomitant isolation of enriched pathogen DNA is an increase of the PCR analytical sensitivity of up to 40,000-fold compared to conventional total DNA isolation.
In total, 6 kits are available for the targeted pathogen DNA isolation from clinical samples. MolYsis™ Basic, MolYsis™ Basic5 and MolYsis™ Basic10 supply pre-treatment solutions of samples for the removal of host DNA and enrichment of pathogen cells from different volumes, respectively. The kits can be combined with any commercial DNA extraction and isolation kits, manual or automated. MolYsis™ Complete5 and MolYsis™ Complete10 are the complete solutions, including host DNA removal, pathogen enrichment, DNA extraction and purification from ≤ 1ml and 5ml or 5 to 10ml sample volume. MolYsis™ Plus enables the extraction of pathogen DNA and removal of inhibitors from grown blood cultures ready for PCR analysis.