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Kits - DNA-free PCR Reagents

DNA-free   |  Highly active   |  Ultra-sensitive bacteria detection   |  Suitable for Real-time PCR

There is a strong risk of false positive signals as a cause of unspecific amplification from contaminating DNA in PCR reagents, including the polymerising enzyme, primers and dNTPs.

Molzym’s purification technology removes traces of DNA from the PCR reagents and supplies a highly active enzyme in the products. This results in enhanced sensitivity and reliability of bacteria detection in the assays.

A series of kits are especially dedicated to the sensitive and specific detection of bacterial and fungal pathogens in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance. MolTaq 16S/18S is the Taq DNA polymerase of choice if you experience contamination problems with other standard Taqs. Mastermixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, MgSO4 (3mM final) and BSA. Mastermix 16S/18S Basic and Mastermix 16S/18S Dye is available for assays using custom primers, Mastermix 16S Primer and Mastermix 16S Complete for universal detection of bacterial DNA, and Mastermix 18S Primer and Mastermix 18S Complete for universal detection of fungal DNA in samples by PCR and Real-Time PCR, respectively. The mastermixes are 2.5x concentrated.

Productinformation:

Product Application  OrderNr. Size Price Shop
MolTaq 16S/18S
DNA-free thermostabile DNA polymerase incl. PCR buffer 1.5 mM Mg 2+ final conc.
bacterial and fungal DNA detection P-019-0100  100 Units  104,-  
P-019-0500  500 Units  446,-  

Mastermix 16S/18S Basic
Premixed PCR reagents for use with custom primers

custom PCR assays  S-040-0100 100 React. 290,-  
 S-040-0250  250 React.  625,-  
S-040-1000 1000 React. 2.250,-  
Mastermix 16S/18S Dye​
Premixed PCR reagents and fluorescent dye, for use with custim primers 
custom Real-Time PCR assays  S-030-0100 100 React. 290,-  
 S-030-0250  250 React.  625,-  
S-030-1000 1000 React. 2.250,-  
Mastermix 16S Primer
​Premixed PCR reagents and universal 16S rDNA primers
eubacterial PCR assay​  S-021-0100 100 React. 320,-  
 S-021-0250  250 React.  725,-  
S-021-1000 1000 React. 2.500,-  
Mastermix 18S Primer
​Premixed PCR reagents and universal 18S rDNA primers
panfungal PCR assay​        
Mastermix 16S Complete
​Universal 16S rDNA assay for detection of bacteria
eubacterial Real-Time PCR assay S-020-0100  100 React. 320,-  
S-020-0250  250 React.  725,-  
S-020-1000 1000 React. 2.500,-  
Mastermix 18S Complete
  
panfungal Real-Time PCR assay        
       
       
Set of sequencing primers    S-775-100  100 React. 20,-  
DNA-free water, PCR grade DNA-free water, 10x 1.7ml  P-020-0003 10x1.7 ml 89,10  

 Preise excl. Ust. und ohne Trockeneispauschale!

 

ahn

brand

eppendorf

gilson

mettler toledo

heidolph

neolab

satorius

systec

thermo scientific

zeiss