Markers
M3 Marker for Spreadex® Gels
The M3 Marker was specially designed for precise size determination of unknown DNA fragments on Spreadex® gels. The complexity of the marker provides both, a suitable number of reference fragments for each Exclusion Limit (EL 300 to 1200) and its separation pattern matches the resolving power of Spreadex® gels. The marker contains over 50 DNA fragments in the size range from 50 to 622 bp.
The fragments were produced by HaeIII digestion of pBR322 (blunt end), and Mspl and Hhal cleavage (overhangs). On Spreadex® gels, all the fragments migrate in the expected order according to their length.
M1 Marker
The M1 Marker was optimised for precise size determination of unknown DNA fragments on GMA gels, 12% and 9% Poly(NAT)® gels, as well as PCR CheckIT™ gels.
DNA fragments were obtained by digestion of pBR322 with MspI restriction enzyme.
Technical specifications for M1 and M3:
Volume 50 µl
Loading 1 µl for EtBr staining
0.2 µl for SYBR stained gels
Storage -20° C
Markers Ordering Information:
| P/N | Product | Quantity |
|---|---|---|
| 3203 | M3 Marker | 50 loadings |
| 3204 | M1 Marker | 50 loadings |
Buffers
- Running Buffer
- Sample Loading Buffer
- PCR Loading Buffer
- 0.2M TMAP (tetramethylammonium phosphate)
- Solid Phase Extraction Buffer
- Oligo Electroelution Buffer
Buffer Ordering Information:
| P/N | Product | Quantity |
|---|---|---|
| 3031 | 40 x Running Buffer Stock Solution, 1.2 M TAE buffer | 1 l (20x50ml) |
| 3033 | Sample Loading Buffer 10 ml | 10 x 1 ml |
| 3034 | Sample Loading Buffer 50 ml | 50 x 1 ml |
Destaining Solution
After incubation in a solution containing an intercalating dye, DNA gels are usually destained in water. The Elchrom Scientific destaining solution speeds up the destaining process and reduces gel background. This is particularly eminent in the case of Spreadex® EL 300-500 gels and 12% Poly(NAT)® gels if stained with SYBR Green or SYBR Gold.
Typically, gels are destained for 20 - 30 min.
The destaining solution is available as a 100 x concentrate.
Destaining Solution Ordering Information:
| P/N | Product | Quantity |
|---|---|---|
| 3037 | DST, Destaining Solution 100x | 50 ml |
